Coding

Part:BBa_K2116053:Design

Designed by: Andreas Zingg   Group: iGEM16_ETH_Zurich   (2016-10-19)


CAT-UPRT Fusion with sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1740
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1060
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

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References

Melançon, Charles E., and Peter G. Schultz. "One plasmid selection system for the rapid evolution of aminoacyl-tRNA synthetases." Bioorganic & medicinal chemistry letters 19.14 (2009): 3845-3847.